Two children were killed and three adults were injured as the result of a five-vehicle crash on Interstate 65 on Sunday.

Nadir Gillis, 9, and Naziya Gillis, 7, both of Camp Lejeune, N.C., were killed when the 2010 Dodge Challenger in which they were passengers was hit by a semi-truck at approximately 10:50 p.m., three miles south of Atmore, according to Kevin Cook, public information officer for the Alabama Department of Public Safety.

The driver of the Challenger, 28-year-old Isom Hodges, and another passenger, Labrica Hodges, 30, both of Camp Lejeune, were transported to a local hospital for treatment of their injuries.

William C. Rousey, the driver of a 2004 Nissan also involved in the accident, was transported to Sacred Heart Hospital in Pensacola. The truck driver, 57-year-old Terry Allen Wyatt of Andalusia, was not injured, Cook said. There were no injuries to the drivers or passengers of the other two vehicles in the crash.

The wreck closed a portion of I-65 and south-bound traffic was rerouted to Jack Springs Road and on to Industrial Drive earlier in the day.

Troopers are still investigating.